ANAESTHESIA
Fentanyl (0.01 mg/100g BW) + Droperidol (0.5 mg/100 g BW)
Induction dose: 0.4 ml/rata IM
Maintenance doses: 3 doses IM of 0.1 ml (prior each 3 anastomosis)
SURGICAL PROCEDURE
Middle laparotomy to expose the left kidney.
Careful ureter dissection only handling its surrounding fat. Divide the ureter close to the kidney thus preserving its whole length.
Left kidney nephrectomy.
Dissection of the abdominal vessels and occlusion with vascular clamps following the sequence:
1. cava caudal
2. cava cranial
3. aorta cranial
4. aorta caudal
If subsidiary vessels were between the clamps, tie them with 6-0 monofilament.
Make the arteriotomy in the aorta the same size as the aortic patch.
Care should be taken not to twist the patch.
Vascular Anastomosis: Artery and Vein
Once the vascular anastomosis is completed, the clamps are removed in the following sequence to allow the restoration of natural blood flow in the kidney:
1. aorta cranial
2. vena cranial
3. vena caudal
4. aorta caudal
Check the anastomosis for bleeding.
Put in extra-stitches if necessary.
RIGHT NEPHRECTOMY
After checking for vascular and ureteric anastomosis, and the correct reperfusion of the kidney graft, perform the right native nephrectomy ensuring to tie the right ureter
ENDING SURGERY
Inject 1ml antibiotic IM (Ciprofloxacine, 2mg/ml) and stitch up the laparathomy with 4-0 absorbable monofilament.
Inject 0.1ml Naloxone IM to revert anaesthesia.
The rat awakens immediately.
Assess the established attention to the rat (supervision protocol) to ensure its welfare
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